Energy Situation and PESTLE Analysis

Question: Talk about the Energy Situation and PESTLE Analysis. Answer: Presentation Denver International Airport that is otherwise called DIA DEN is set in Denver, Colorado, United States. DIA is the greatest air terminal in the US. Alongside this, it is the 6th busiest air terminal in the US and eighteen busiest air terminals in everywhere throughout the world. Denver has the third driving residential association organize inside the country (DEN. 2017). Denver air terminal is the principle place for Frontier Airlines just as Great Lakes Airlines. It is the fourth predominant place for United Airlines. Also, it is the main air terminal in the US that has executed an ISO 14001 authorized natural administration framework to cover the total air terminal adequately. A PESTLE examination is a successful showcasing instrument that is utilized by the business associations to assess the full scale ecological components that impactsly affect the associations. Alongside this, it is additionally utilized by the business firms to follow the earth in which they are working or likewise ready to work their organizations. A PESTEL investigation is just significant to examination large scale natural factors, for example, political, financial, social, innovative, legitimate and ecological components (Wilcox and Jenkins, 2015). Denver International Airport utilizes this device to find the outside components that affect its tasks. The PESTEL investigation of Denver is given as underneath: Political Factors: Political Factors affect the associations with respect to government guidelines just as legitimate issues; and furthermore depict both formal casual principles in which business firms are obliged to work their organizations. Political strength, government approaches, exchange limitations, charge strategy, ecological guidelines, business work law, duties, etc are the significant instances of these political variables. Political elements may shift from one country to other country. Be that as it may, the air terminal industry is comprehensive and spreads the whole globe (Prather, 1998). Alongside this, concurring the report, the flying business has been created roughly $709 billion in the year 2016. The administration guidelines of the country focus on concerns, for example, quiet security, wellbeing, and some different issues. In addition, in the assembled expressed, in 1978, the deregulation happened that decreased the limitations inside the business. Deregulation canceled the principles just as guidelines those were related with the costs and section of the air terminal association into household markets. As an outcome, the prevalence of the business expanded. The expanded fame additionally upgraded the incomes of the air terminal industry in the United States. Then again, it ought to likewise be noted down that, the situation isn't the comparative in all the business sectors. It is a direct result of certain business sectors are exceptionally managed in the United States. The exceptionally managed markets lessen the incomes of the associations (Haberberg and Rieple, 2008). In addition, in such markets, the worldwide flights are exceptionally directed instead of household (national/home) flights. Likewise, Denver International Airport (DIA) additionally works its business in an intensely controlled world of politics. It is a result of in carrier industry travelers are more important than aircrafts and their security is the principle worry of the legislature. At the end of the day, it likewise can be said that, traveler security must be predominant for the carrier associations. By thinking about the patient security, the US government has set up exacting guidelines for the tasks of carrier associations (Schloh and Hardship, 1996). As a result, unmistakably, DEN works in an exceptionally controlled political environment of the country. The United States has severe guidelines for the air terminals and aircrafts. It is a result of such associations face various dangers that may reason for mishaps, irremediable harms, and passings of travelers. Thus, the US government has been made and executed heaps of arrangements, rules, and guidelines to ensure travelers in inadvertent cases. In this way, Denver needs to satisfy all the guidelines and guidelines to work its business inside the country. Then again, the legislature of the country additionally offers loads of relaxations to air terminals and carriers. The legislature is severe for the patient security not for the aircraft business of the country. It is a direct result of carrier offers various advantages that improve the economy of the country (Williams and Curtis, 2007). Therefore, the administration of the US produces an incentive by being partners of the carriers organizations. The world of politics has been loose in certain business sectors just to lead association in an efficacious and a progressively extensive way. The alliance between the administration and aircraft organizations isn't valuable for these two however advantageous for travelers moreover. Alongside this, modern guideline and world of politics in a planned way give advantages to everywhere throughout the world. In this way, it is clear that administration guidelines just as political variables of a country assume various significant jobs to choose the development and plenitude of the aircrafts organizations inside country. In this manner, clearly Denver air terminal works its business in a very managed political milieu. DIA is likewise getting a charge out of bunches of advantages because of the connection between the US government and the carrier business. The association has helped the benefit and incomes of the carrier organizations (Kerzner, 2012). In this manner, Denver International Airport is doing great so as to heighten its relationship with the legislature of the country. Financial Factors: Economic variables have various huge effects on the development and achievement of the business associations. For case, monetary development, swelling rates, financing costs, interstate charges, trade rates, embargoes, downturn, joblessness, wage rates, typical cost for basic items, credit accessibility, etc are the major monetary elements. Further, these monetary elements can be separated into two variables like: full scale financial and small scale monetary components. These the two variables accentuation on all the occasions those affect the interior just as outside monetary condition. The miniaturized scale financial occasions are identified with the practicality and inside adequacy of the business (Liebhold, Work, McCullough and Cavey, 2006). Additionally, the large scale monetary occasions contain loan costs, swelling rate, downturn, conversion standard, joblessness, working hours, financing accessibility, etc. Then again, financial elements of a country have enormous impact on the carrier organizations. The US carrier industry has seen various descending spirals. The air terminals and carriers of the country are as yet not recouped from the results of the 9/11 assaults. Alongside this, Denver air terminal is influenced by the ongoing downturn just as moderate monetary development of the country. The monetary insecurity of the country decreased the incomes of the DIA and other comparative organizations. Additionally, on account of the downturn, Denver air terminal likewise decreased its expenses to achieve upper hands over the contenders (Whitehead, 2012). Also, the drawn out downturn, oil costs changes, and an approaching worldwide log jam are other upset financial components that are influencing the development and achievement of DIA inside the economy of the country. Denver is attempting its best to oversee and hold its travelers adequately (Schloh and Hardship, 1996). As a result, it has executed a mechanized stuff framework to diminish delays, gather holding up times at baggage claims, decrease carrier work costs, and improve the notoriety in the worldwide market. DIA is additionally managing expanded high fuel costs and working upkeep costs in an adequate way. Aside from this, the downturn had abandoned various beneficial outcomes it. Because of the downturn, air terminals like Denver air terminal have changed their style of working. They likewise improved their administration principles and furthermore discounted their costs to draw in an ever increasing number of clients. The air terminal is a base of the entire aircraft indust ry. In current, the business is altering its business according to the changing monetary states of the country (Zalengera, Blanchard, Eames, Juma, Chitawo and Gondwe, 2014). Be that as it may, a great deal of development is assessed in the up and coming years; and because of the development of aircraft industry, the eventual fate of Denver air terminal can be seen splendid later on timespan. Social Factors: Social variables or socio-social elements investigate the social condition of the market, and measure determinants, for example, socioeconomics, social patterns, populace examination, etc. Alongside this, these components include the common conviction just as mentalities of the masses. These socio-social variables contain populace development, social angles, social desires, wellbeing cognizance, populace elements, age appropriation, vocation perspectives, standards, an Earth-wide temperature boost, and so forth. These social elements center around the security and wellbeing of the masses (Ho, 2014). What's more, high patterns in socio-social variables affect the interest and flexibly for the items or administrations of the organizations. Business associations may change their administration methodologies to take focal points of these social patterns for the development and achievement of the business. Then again, it is notable that Denver International Airport is the greatest air terminal in the United States. It thinks about social variables to improve the matter of the carrier business. Additionally, air terminals and carriers create social qualities for the development and achievement of the organizations. There are bunches of enterprises that gain benefits from the carriers (Wilcox and Jenkins, 2015). Air terminals and aircrafts are significant methods for transportation for the individuals and business associations. The two of them are profoundly affected by the social factors and powers too. In this circumstance, the DIA has scaled down its costs to address the issues and requir

A Guide to Ethnomethodology

A Guide to Ethnomethodology Ethnomethodology is the investigation of how individuals utilize social collaboration to keep up a progressing feeling of reality in a circumstance. To assemble information, ethnomethodologists depend on ​conversation examination and a thorough arrangement of procedures for efficiently watching and recording what happens when individuals interface in characteristic settings. It is an endeavor to arrange the moves individuals make when they are acting in groups.â Causes of Ethnomethodology Harold Garfinkel initially concocted the thought for ethnomethodology at jury obligation. He needed to clarify how the individuals sorted out themselves into a jury. He was keen on how individuals act specifically social circumstances, particularly ones outside of the every day standard like filling in as a juror.â Instances of Ethnomethodology A discussion is a social procedure that requires certain things with the goal for members to recognize it as a discussion and prop it up. Individuals take a gander at one another, gesture their heads in understanding, ask and react to inquiries, and so forth. In the event that these techniques are not utilized accurately, the discussion separates and is supplanted by another kind of social circumstance.

The History of the Thematic Analysis Psychology Essay

The History of the Thematic Analysis Psychology Essay Thematic analysis is an analytical technique serving to interpret the contents of texts or human experiences and widely used in qualitative research. In its interpretation of qualitative data, thematic analysis generates themes within the distinguished categories of data. Each category that reflects a recurring feature of the obtained data attributes a code to a corresponding informational block. However, data categorization is thematic analysis pursues the aim of managing the overall volume of unstructured data rather than indicating conceptual issues. In this vein, thematic analysis entails a clear and precise definition of codes that are distinct from one another. During analytical procedures, a researcher refers to the developed code log book to categorize data and allow themes to emerge.[1] In other words, a theme in thematic analysis represents a statement of meaning observed in most of the data. This meaning may be also reported by a minority of participants, but carry a strong emotional power or factual impact on the studied situation. In thematic analysis, themes may be regarded as units formed by deriving and comprising patterns of conversation issues, meanings, feelings, recurring activities. Proverbs, and vocabulary. As such, themes serve to bring together elements ideas, perceptions, or experiences that may seem meaningless when considered separately.[2] In other words, the role of thematic analysis is to summarize the data content and draw relationships between distinct codes to produce a social meaning shared by the examined population sample. Therefore, thematic analysis is an interpretive analytical technique dedicated to revealing new concepts and ideas. Thematic analysis is theoretically flexible and allows answering various research questions without adherence to a specific framework. This analytical approach is mostly beneficial for responding to research questions regarding human experiences, ideas, attitudes, and perceptions.[3] Its focus on motifs and beliefs driving individual behavior fits scholar ship in psychology, social studies, behavioral research, and more applied disciplines like education, health, and clinical research. Psychology is a discipline that seeks to explore and analyze human experiences and behaviors to determine factors and personality traits shaping individual’s behavior patterns. In this vein, qualitative studies represent a significant portion of psychology scholarship when researchers attribute meanings to different behavior types observed or discussed with human participants.[4] In line with the prevailing qualitative research design, psychological studies focus on synthesizing the attained data and interpreting results into meaningful conclusions. In this pursuit, scholars tend to use different names to describe content analysis procedures utilized in their inquiries or using them without a specific description. Regardless of the expressed position, psychologists appreciate the thematic analysis framework for several benefits offered. While examining human observations, feelings, and attitudes, this analytical approach tends to bridge the researcher and participants intellectuall y and emotionally, fostering understanding of the former of motifs and beliefs underpinning behavior of the latter.[5] Moreover, thematic analysis allows combining different research methods that is a substantial advantage for psychological research. For instance, thematic analysis is applicable to processing and interpreting data obtained through both qualitative and quantitative methods. In thematic analysis, interview findings may be complemented by survey results to expand the depth and breadth of the acquired understanding while strengthening validity and reliability of the produced conclusions.[6] This argument is crucial in terms of qualitative research criticism that stresses on high subjectivity and descriptiveness of qualitative research findings. When applied to a single research method, thematic analysis serves to organize the unstructured dataset and embed a systematic approach in the analytical process. In the case of psychological research utilizing several research methods, thematic analysis is useful to generate an insight into the studied phenomenon while proving credibility of the attributed meaning. References Boyatzis, Richard E. Transforming Qualitative Information: Thematic Analysis and Code Development. Thousand oaks, CA: Sage, 1998. Gibson, Will. “Qualitative Research Analysis in Education.” In Educational Research and Inquiry: Qualitative and Quantitative Approaches, ed. Dimitra Hartas, 295-307. London: Continuum International Publishing Group, 2010. Goodwin, C. James and Kerri A. Goodwin. Research in Psychology: Methods and Design. Hoboken, NJ: John Wiley Sons, 2016. Thatchenkery, Tojo and Carol Metzker. Appreciative Intelligence: Seeing the Mighty Oak in the Acorn. San Francisco, CA: Berrett-Koehler Publishers, Inc., 2006. [1] Ibid. [2] Tojo Thatchenkery and Carol Metzker, Appreciative Intelligence: Seeing the Mighty Oak in the Acorn (San Francisco, CA: Berrett-Koehler Publishers, Inc., 2006), 235. [3] Ibid. [4] C. James Goodwin and Kerri A. Goodwin, Research in Psychology: Methods and Design (Hoboken, NJ: John Wiley Sons, 2016), 302. [5] Richard E. Boyatzis, Transforming Qualitative Information: Thematic Analysis and Code Development (Thousand oaks, CA: Sage, 1998), 6. [6] Goodwin and Goodwin, 302.

Capital Punishment Should Be Abolished - 1137 Words

The case for capital punishment Topic: why capital punishment should continue General purpose: To argue Specific purpose: my main aim is to convince the audience that capital punishment should be upheld. In other, the advantages of capital punishment outweigh its disadvantages. Introduction Capital punishment is also known as death penalty and it has been in existence since time immemorial. Throughout history, the death penalty has been used to punish a number of crimes that include murder and other premeditated crimes. However, there has been growing campaigns calling for the abolishment of capital punishment. Many of these campaigns have been spearheaded by religious groups and other interested parties. As a Christian, I am of the opinion that such arguments have no biblical bases because the bible advocates for the application of the death penalty for certain crimes like witchcraft, murder and kidnapping. Whenever the word capital punishment is mentioned, it raises a lot of heat from all the sides of extremist. One side argues that capital punishment should be abolished because it may result in the execution of an innocent person while the other side arguers that capital punishment deters crime. As noted earlier, the death penalty is one of the most discussed topics in the criminal justice system. The death penalty used to be legal in the united states until 1972 when the supreme court in Furman v. Georgia ruled it unconstitutional arguing that it violatedShow MoreRelatedCapital Punishment Should Be Abolished965 Words   |  4 PagesHaesemeyer Advanced Studies English 9 7 April 2017 Capital Punishment Over the centuries, capital punishment has fallen in and out of public support. In several countries, the practice has been overruled by law. In others, it is simply not exercised. More than half of U.S. states still practice capital punishment for capital crimes. Often, innocent people are sentenced to death because of circumstantial evidence. Capital punishment should be abolished in all fifty U.S. states because of the severalRead MoreCapital Punishment Should Not Be Abolished1541 Words   |  7 Pagestime, if a person committed a severe crime, like murder or rape, they were executed to maintain peace in the community and to bring comfort to those who knew the victim. Capital punishment has been used in almost every part of the world, but in the last few decades many countries have abolished it. The issue of capital punishment has been a sensitive topic for nations attempting a careful balancing act between prisoner’s rights and legal defense teams and society’s la ws on cases of extreme gravityRead MoreCapital Punishment Should Not Be Abolished901 Words   |  4 Pagesgovernment (â€Å"Capital Punishment in the United States†). Capital punishment is being debated all over the world whether it is murder or justice for the crime they have committed. Statistics show that murderers often kill again after releasement from prison. The Bureau of Justice gives relevant statistics pertaining to murderers who were released from prison: in 1994 40.7% of murderers were arrested for a new crime within three years of release (United States Department of Justice). Capital punishment shouldRead MoreCapital Punishment Should Be Abolished1115 Words   |  5 Pagesjail time, house arrest, and/or having to pay fines. Crimes that are severe can lead to greater punishment, like spending life in prison. The most severe crimes can lead one to an equally severe punishment known as capital punishment. Capital punishment is the authorization to kill someone fo r the crime he or she has committed. Capital punishment, commonly referred to as the death penalty, should be abolished in all states because it can put innocent lives at risk, it costs millions of dollars each yearRead MoreCapital Punishment Should Not Be Abolished750 Words   |  3 PagesCapital Punishment Should Not be Abolished There are many reasons why the United States of America keeps capital punishment. These reasons include the deterrence theory, the idea of retribution, cost of prisons, and general safety of the public. First of all, I’m sure that you have heard of the deterrence theory. Deterrence is basically the fear of punishment; and even though it doesn’t prevent all crimes, the results are still undeniable. The deterrence theory is when criminals thinkRead MoreCapital Punishment Should be Abolished883 Words   |  4 Pages Capital punishment is the punishment of death for a crime given by the state. It is used for a variety of crimes such as murder, drug trafficking and treason. Many countries also have the death penalty for sexual crimes such as rape, incest and adultery. The lethal injection, the electric chair, hanging and stoning are all methods of execution used throughout the world. Capital punishment has been around since ancient times; it was used in ancient Rome, and one of the most famous people to be crucifiedRead MoreCapital Punishment Should Be Abolished1955 Words   |  8 Pagesit has become apparent that capital punishment should be abolished worldwide. Around t he world, various countries continue to practice this brash, inhumane punishment. The legal system is intended to regulate citizen’s behaviour which aims to provide a smooth functioning society. When someone is sentenced to death, the legal system loses the â€Å"fair† aspect of the proceedings, as under no circumstances is the death penalty â€Å"fair†. Capital punishment should be abolished because it violates Section 12Read MoreCapital Punishment Should Be Abolished1135 Words   |  5 Pagesname -unknown. Nobody should ever have to hear these words. Especially coming from their own state the one that is supposed to protect their rights no matter what. Capital punishment should be abolished from the United States. The number one goal of the constitution is to protect the life of an individual and the death penalty passes by this. The death penalty is an easy way out of the crime, it is expensive, philosophers and even psychologists disagree with capital puni shment and most important itRead MoreCapital Punishment Should Be Abolished Essay1293 Words   |  6 PagesApproaching the topic of capital punishment is difficult when looking at society as a whole. In the opinion stated by my class group, capital punishment should be entirely abolished due to the possibility of mistakes while sentencing. This idea relies on the basis that capital punishment has absolutely no purpose or benefit. Members of my group mistakenly used a utilitarianist point of view to argue their case, even though this view can be stretched to fit almost any argument. This paper will exploreRead MoreCapital Punishment Should Be Abolished1439 Words   |  6 Pagesto outlaw capital punishment in America completely. We are meant to be better and bigger than this then why do w still have this barbaric law in practice it is inhumane, morally wrong and expensive. In my paper I will try to cover the taboo topic of capital punishment and why it should be abolished because if we as a society want to grow and advance in a sophisticated society we must abolish the death penalty all across the land. To begin with I must explain what capital punishment really is and

Find Out Whos Concerned About University of Chicago College Essay Samples and Why You Should Listen to Them

Find Out Who's Concerned About University of Chicago College Essay Samples and Why You Should Listen to Them Up in Arms About University of Chicago College Essay Samples? There's, clearly, a limit on the range of pages even our very best writers can produce with a pressing deadline, but usually, we figure out how to satisfy all the clients seeking urgent assistance. Furthermore, the high quality and originality of the item is guaranteed. At times, the best method to learn and understand new information is by way of seeing and understanding work which is already completed. You don't have to be worried about your personal details that could be viewed, as we handle the matter on a safe network. I have always enjoyed riding in cars. Explain the auto connection better. Descriptive essay about an auto accident. The exact same is true in the United States of america. Thus, State University isn't only the ideal location for me, it is the sole spot for me. On top of its growing cultural and ethnic diversity, it is becoming a master at creating a niche for every student. The University of Chicago is known for quirky and elaborate essay prompts. Who Else Wants to Learn About University of Chicago College Essay Samples? Last, don't neglect to connect your very own past experiences and accomplishments to the resources you decide to highlight. Student loans aren't free money and has to be paid back. The findings reveal that in Malaysia, it's quite complex to demonstrate negligence in instances of road accidents because proving a case is contingent on the elements to be proven. Yet, three decades later, not one of that transpired. The application text needs to be effective to put in the college. All you need to do is to locate reliable sources of information which offer writing tips. You have to construct a good outline before you write. Inside this section you'll also find more comprehensive info about each stage in the application approach. 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GFP protein Free Essays

string(97) " the low salt beaker and in the column chromatography, causing GFP to spread down the container\." Green Florescent Protein, abbreviated as GFP, is a protein composed of 238 amino acids that is commonly found in mnemiopsis, comb Jelly. It has a major wavelength at 396 nm and a minor one at 475 nm. GFP is what gives mnemiopsis their bright green florescent glow. We will write a custom essay sample on GFP protein or any similar topic only for you Order Now ultraviolet light, or blue light, is necessary to see the florescent glow of this protein. GFP is an irregular protein because It Is highly resistant to denaturation by temperature and PH. It can survive In temperatures up to 98 degrees and has a pH of 12. 2 due to Its complex exterior, called the beta barrel. At an pH higher than 12. It denatures. It also has an Isoelectric point at 5. 3. The peripheral beta barrel cannot be digested or broken apart by protease because of the strong bonds holding It together. The beta barrel protects the chromophore, which Is the substance which gives GFP Its green glow. When CFP Is extracted from the plasmid of an E. Coll or from a Jellyfish, It contains an array different contaminants making it difficult for scientist to do experiments with GFP. A procedure in purifying GFP from a crude cell extract to nearly 100% GFP so that it can be analyzed and used in scientific experiments and research is necessary. The goal is to ptimize each protocol used to purify crude GFP. Methods Ammonium Sulfate Precipitation To purify the crude samples of GFP, the ion exchange method separates substances inside the test tube by similar charge. A sample of crude GFP of 7. 5 mL in a plastic tube was used for the experiment. Knowing that 43. 6 grams of ammonium sulfate in a 100 mL solution yields a 70% percent saturated solution, the proportion 43. 6g 11 00 mL=x/7. 5 mL was used to determine that 3. 27 grams of ammonium sulfate needs to be added to the experimental sample. After adding the ammonium sulfate, the solution was stirred gently to prevent frothing. Once most of the solution is transferred, the tube was placed on a triple beam balance along with another tube that went through the same process. The centrifuge was set at 15,000 rpms for 15 minutes so that the hydrophobic materials will separate and become the supernatant while the GFP pellet will remain behind. Once the 15 minutes elapsed, a new pipette was used to remove the supernatant, leaving behind the pellet of GFP and hydrophilic contaminates. To remove the hydrophilic substances, 5 mL of 4 molar ammonium sulfate and 15 mL of 10 mL tris at a p of 8 was added Into the oak ridge entrifuge test tube. The solution Is then put Into the centrifuge at 15,000 rpm for 15 minutes again. Once 15 minutes has passed, the supernatant, containing the GFP, was removed by a pipette and put In a microfuge. Hydrophobic Interaction Hgure yaropnoDlc Interactlon set up One molar ammonium sulfate was added to the column to wash the sample. Adding 1 molar ammonium sulfate washes the sample because a high salt concentration increases hydrophobicity of the GFP and the buffer, causing most of the GFP to be at the very top of the column. Substances that are hydrophilic get flushed out of the olumn while the more hydrophobic substances stay in the container. After the column has been eluted with 1 molar ammonium sulfate, the tris buffer is added to the ammonium sulfate to dilute it into . 5 molar ammonium sulfate. The volume of 1 molar ammonium sulfate inside the oak ridge centrifuge test tube is the volume of the tris buffer that will be added. After the column chromatography has been flushed with . 5 molar ammonium sulfate, more hydrophobic substances will be flushed out since the hydrophobicity of the tris buffer and the GFP has decreased. This causes the GFP to spread out in the column. Finally the amount of . 5 molar ammonium sulfate is diluted with tris buffer to . 5 molar ammonium which should cause most of the GFP to be flushed out of the column along with other substances that are very hydrophobic. While this experiment is going on the liquid that comes out of the column is collected in multiple test tubes. These test tubes contain GFP and other contaminants. The solutions are than read by a spectrophotometer. Each test tube wil l be tested by the spectrophotometer so that a graph can be made. Anion Exchange Figure 2: Siphon Bridge set up for Anion Exchange Figure 3: Centricon Test Tube In order to use anion exchange, the starting condition of the sample needs to be in a low salt solution. However after the GFP had gone through hydrophobic interaction, it was in a high salt solution. Before facing this dilemma, the fractions were pooled by centricon which decreases the overall sample volume by removing some buffer and salt solution. This greatly increases the GFP concentration in the samples. The fractions are placed in the centricon and then into a centrifuge for 25 minutes at 3,000 rpm to be separated by size. The large proteins stay in the entricon while buffer and salt solution goes into the plunger. To reduce the concentration of salt in the GFP sample, the sample is diluted 10 folds. Since the amount of GFP that was restored was 18 mL, 162 mL of tris buffer needed to be added. The diluted GFP is then put in the chromatography container, containing positively charged DEAE which is attracted to the GFP at a low salt concentration. After the GFP has been completely filled, the column is connected to a beaker that contains a low salt concentration. the low salt concentration beaker is connected to a high salt concentration beaker. As one drop of low salt solution goes into the chromatography column, one drop of high salt solution goes into the low salt solution. Gradually the salt concentration increases in the low salt beaker and in the column chromatography, causing GFP to spread down the container. You read "GFP protein" in category "Papers" The eluted GFP dripped out of the column chromatography to be collected in test tubes. I nree pnase partltlonlng Figure 4: Precipitate of GFP. T-butanol is one top while contaminates are on bottom GFP then went through three-phase partitioning, also known as TTP. The fractions taken after an anion exchange was 15 millilieter. Ten ml of 4 M ammonium sulfate was added to this volume to increase the salt concentration of the solution to 1. M, which is about 40% salt saturation. Twenty-five milliliters of t-butanol was added then added which was the same amount of ammonium sulfate and GFP in the container. The container was then placed in the centrifuge for ten minutes at 4600 RPM, causing the mixture to split into three layers; butanol on top, GFP in solution on the bottom, and precipitated contaminants in-between. The top layer of butanol and disk of precipitate were taken out. The volume of GFP solution was again matched in utanol and the container went into the centrifuge again. An aspirator was used to extract the GFP into a microfuge. . 6M ammonium sulfate was then added to the microfuge and the container was placed in a micro centrifuge for one minute at 13,000 RPM. Butanol and other contaminants that had not been take out previously formed a disc, was then taken out with an aspirator and the remaining GFP was then left in the microfuge. HPLC Figure 5: HPLC basic layout After the sample went through three phase partitioning, it was put through the High Performance Liquid Chromatography for a final purification. First liquid was put into the HPLC to clean out any previous GFP inside the loop of the HPLC and the column of the HPLC. Then, GFP in the microfuge was sucked into an injector to be put into the HPLC. Pushing the top of the injector slowly, GFP entered into a loop inside the HPLC. Once the GFP was placed in the loop, a knob was turned clockwise to the word lock. The GFP was then sent to the column where it was purified further by size through the minuscule beads. About 6,000 pounds of pressure per square inch was produced by the HPLC to push the GFP through the beads. While this was occurring, a pectrophotometer connected to the HPLC read the wavelengths of substances. Near the 396 nm wavelength, GFP was collected in a microfuge tube. A UV light was held near tne exlt 0T Results e HPLC to measure tne amount ng sample. Graph 1: Results of the sample after HIC at a wavelength of 395 nm Graph 2: Results of the sample after HIC at a wavelength of 280 nm Graph 3: Results of the sample after HIC of the entire spectrum Seventeen test tubes were received after the HIC purification process. A blank consisting of tris buffer and ammonium sulfate was sampled in the spectrophotometer against liquid from each of the seventeen test tubes. Graph one represents the sample after HIC at a wavelength of 395 nm while graph two Results shows the results after HIC at a wavelength of 280 nm. After HIC, the fractions 12 to 16 were chosen for their purity and recovery of GFP. Graph one show the amount of GFP in each fraction number while graph two shows the total amount of protein in each fraction number. Graph three shows the spectrum of the entire sample. Graph 4: Results after Anion Exchange at a 397 nm wavelength Graph 5: Results after Anion Exchange at a 280 nm wavelength Graphing 6 Thirteen test tubes were collected from the Anion Exchange purification process. This time the samples were blanked against tris buffer at 8. 0 pH and 0. 5 molar sodium chloride. Graph four shows results of the Anion Exchange at a 397 nm wavelength and graph five shows the results after Anion Exchange at a 280 nm wavelength. Once again, the graph at a 297 nm wavelength shows the amount of GFP while the graph at a 280 nm wavelength shows the amount of total protein. Graph six represents the results of the entire spectrum. The GFP peak was a lot more visible. Step Iotal sample (mL Abs (280) Total Protein Abs (397) GFP Ratio Crude sample 120 1600 . 25 At-ns042- 20 1 . 61 . 9 118 HIC 18 . 28 . 173 . 618 15 . 126 . 130 1. 03 3 Phase Partitioning . 01 n/a . 75 . 243 . 257 1. 06 Table 1: This is the overall data table. The second column shows the total volume at the start of each purification step. The following two columns are the peaks of the graphs at those wavelengths. The last column represents the ratio of GFP to the total Protein. The most desirable ratio is 1. 2 5. Dlscusslon The first method in purifying the crude GFP was using the ammonium sulfate precipitate. When ammonium sulfate is placed in water, it dissociates into ammonium (NH4+) and sulfate ions (S042-). Water, composed of two hydrogen ions and one oxygen ion, is a polar molecule because the oxygen has a high electronegativity. Oxygen has a greater affinity making the oxygen portion of water negative and the hydrogen portion of the water positive. The dissociated positively charged ammonium ion is allured to the negatively charged oxygen while the negatively charged sulfate ions are attracted to the dissociated positively charged hydrogen. The attraction between the ammonium sulfate and the water was so strong that the GFP and other proteins were left unoccupied, causing them to precipitate. When GFP in the 70% salt solution was placed into the centrifuge, substances such as DNA and RNA was removed because they became part of the supernatant. At a 70% salt concentration, only hydrophilic substances stay in solution while the more hydrophobic substances precipitate. When the GFP in a 25% solution of salt was placed in the centrifuge, the GFP and other substances went back into solution because there not enough water was occupied by the salt. Before the GFP is placed in the centrifuge, it must be balanced with another centrifuge with the same weight and the two containers must be placed across from one another. This is vital because the centrifuge needs to be balanced when it is rotating at an incredibly fast speed. Failure to have balanced centrifuge containers can result in a broken centrifuge and loud sounds. Also when mixing the GFP with salt, it is important not the shake the container or frothing will occur, making it difficult to transfer the solution in to an oak ridge centrifuge tube. The second purification procedure that GFP underwent was hydrophobic interactions. During this purification, GFP binded to the non-polar Phenyl Sepharose beads because of its non-polar and hydrophobic traits. However the water in tris buffer is strong enough to separate the attraction between GFP and the Phenyl Sepharose. Therefore a high salt concentration is necessary to occupy the water so that the GFP and the Phenyl Sepharose to be attracted together. At a high salt concentration, GFP with bind easily to the Phenyl Sepharose since very little water molecules would interfere with the attraction and at a low salt concentration, GFP would not bind easily to the Phenyl Sepharose because tnere wlll De a lot 0T unoccuplea water molecules tnat wlll De aDle to InterTere wltn the GFP and Phenyl Sepharose attraction. Before the experiment, ten millimolar tris buffer at a pH of 8 was used to clean the column in order to keep the pH stable and to wash away the salt, ammonium sulfate, in the column. Removing the salt is vital because the buffer that once surrounds the salt will be allured to the hydrophobic benzene and to the hydrophobic patches on the GFP. Since the hydrophobic patches of the GFP are already filled, they will be flushed out, leaving mostly beads of benzene and the 10 millimolar tris buffer at a pH of 8. Once the column has been clean, it needs to be equilibrated so that the salt concentration is the same through the olumn. The step gradient used, started ata 1 molar ammonium sulfate concentration and was halved until a . 25 molar concentration to separate substances by hydrophobicity. The third purification procedure was anion exchange. In this procedure, GFP and other contaminants are separated by charge. The beads in the containers are different from the beads from the hydrophobic interaction because on they have a different chemical called DEAE which makes them positively charged. GFP has both protons and electrons on it which is why it was not easily attracted to the DEAE, which is why the GFP is put in a basic solution. Ata high pH, the amount of negatively charged hydroxide increases and these hydroxides are allured by the protons on the GFP. The protons are than neutralized, making GFP a negatively charged molecule. The isoelectric point of GFP is at a pH of 5. 3. Ata pH higher than 5. 3, it is negatively charged and when it is at a pH lower than 5. 3, it is positively charged. Once the column chromatography is filled with GFP and connected to a beaker of low salt which connected to a beaker of high salt, anion exchange occurs. As the salt concentration increases, the GFP slowly spreads down the column and eventually out f the column into test tubes. Between the HIC and the Ion exchange chromatography, the sample the fractions were pooled and put in a centricon causing the GFP concentration in the samples to increase. This occurred because the ultrafilter only allowed particles smaller than protein to go in to the pusher. The large proteins stay in the centricon while buffer and salt solution goes into the plunger. The sample of GFP was also diluted 10 folds because the sample needs to be in a low salt solution to use anion exchange and after the GFP had gone through hydrophobic interaction, it was in a high salt solution. The anion exchange method creates a continuous salt gradient because as one drop of low salt solution goes into the column chromatography, causing GFP to spread down the container. The follow procedure was the three phase partitioning purification. T-butanol and 1. 6 molar ammonium sulfate were essential for this procedure. T-butanol has a low density causing in to stay above the GFP solution. In addition it has an attraction for water and other hydrophobic substances causing 5 mL of water to be drawn out of the GFP sample and precipitated substances to float between the t-butanol and the GFP sample. Fresh t-butanol is necessary after removing the old t-butanol with the contaminants because at that point, the salt concentration had increased since water was drawn out. was aDle to De preclpltatea Decause 0T tne nlgn salt concentration. The final procedure for purifying GFP was using the HPLC which separated substances by size. The beads used in the HPLC column are miniscule and porous. The pours on the beads give substances of the same size more opportunities to leave the HPLC at the same time. Since the beads are so small, high pressure is needed to push the GFP sample through the beads. Naturally, smaller substances will exit the HPLC first while larger materials will exist last. In all scientific experiments room for error is unavoidable. During the HIC, IEX, three phase partitioning, and the HPLC, amounts of GFP were lost due to the GFP sticking to a container, a pipette, and even spills. During the HIC some of the GFP was lost due the overflowing the test tubes with liquid exiting the column. During the HPLC some GFP was lost because not all GFP dripping out of the HPLC went in to the microphage. Other errors include letting the column dry because the liquid was not dded to the beaker about the column. During the spectrophotometer runs, the blank was no inserted correctly causing the reading of the GFP to be incorrect. In addition, the order in which the GFP samples were suppose to be placed in the spectrophotometer was messed up. Judging from the overall purification table, table 1, the purification was quite successful. Originally, the ratio was only . 25, but by the end of all the purification procedures, it obtained a ratio 1. 06. A 1. 25 ratio is most desirable and through the purification, the ratio was nearly reached. The anion exchange, three phase artitioning, and the HPLC purification were the most impacting procedures. The anion exchange greatly increased the purity of the crude sample compared to the HIC purification. The three phase partitioning and HPLC purified the GFP even more. Some improvements to the protocols would be to start with the anion exchange purification so that overall, the salt solution would go from a low salt concentration to a higher salt concentration. This also eliminates the need to dilute the solution. In addition, an automatic machine could be used to shift the test tubes that collect the iquid exiting the columns to prevent overflowing test tubes and the risk losing GFP. GFP is unique because of its florescent glow. This glow can be used as a marker or an indicator. If a glowing marker could be placed on infectious cells such as tumor cells or cancerous cells, it would revolutionize the treatment of these diseases because doctors will be able to track where the harmful cells are. In addition, if it is possible to trigger the florescence of GFP with UV light, it can eventually be used in light bulbs to produce light. GFP light bulbs would last for an incredibly long time ince they are very resistant to denaturing. In addition, in vehicles, GFP can be mixed in the motor oil, transmission oil, power steering oil, air conditioning oil, and other oils so that if a leak occurs in a car, it can easily be spotted by shinning UV light on the car. The purification of GFP can lead to endless new innovations in electrical engineering, automotive repair, and curing deadly diseases. How to cite GFP protein, Papers

The Business Network free essay sample

How are these two approaches different? Does it depend on the state of the economy which one should be used? Why? Before the dot-com debacle Cisco Systems used â€Å"push† approach – a lot of inventory was made, and it was based on best-guess forecasts. This approach was not bad when economy was strong and there was huge demand and there was no need to predict precisely the inventory. When during the recession of the economy Cisco Systems implemented â€Å"pull† approach, which rely on precisely planning of inventory. The second approach allows having free costs because of reducing inventory and also freeing up working capital. 2. What are the different elements that need to come together to bring supply chains to the optimal levels needed by these companies? What role does IT play? In order to bring supply chains to the optimal level company needs to coordinate supply chain operation system with IT technologies. We will write a custom essay sample on The Business Network or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Also, in order to work in appropriate manner system needs to get and adapt information from marketing, finance, sales and supply department. IT plays a very important role: its connect the whole system, coordinate it and adapt. . How are the approaches to inventory management taken by O’Reilly Auto Parts, on one hand, and Cisco Systems and Black amp; Decker, on the other, different? O’Reilly Auto Parts uses inventory as a competitive differentiator and try to increase customer service levels and replenish inventory on a nightly basis, while at the same time managing an increasing number of products. Also its strategy relies on partnership between the supply chain operation and IT. Black amp; Decker chose another strategy: they try to lower inventory levels to free up working capital.

The effects of making employee salaries public

The effects of making employee salaries public We’re living in a rapidly evolving world where almost everything is done online and the very notion of privacy seems to be evaporating. Most of us are growing increasingly more comfortable having our lives made public through a variety of social networks. This new open and public approach to sharing information is affecting companies as well. How so? Many companies are embracing the notion of complete public transparency and disclosure in ways they never have before. Everything is potentially on the table for being made public, including employee salaries- something that up until recently has long been held in the strictest of confidence. Let’s dig deeper into this concept of employee pay transparency, and how it’s affecting the status quo.The idea behind making employee salaries public is an arguably noble one- more and more companies are seeking to embrace the philosophy that being open and honest with their employees about all things is an effective way to for ge more progressive, sincere, and honest employee/employer relationships. It can also help address some unfortunate inequities in compensation that women and minority groups sometimes face, an issue that gets inadequate attention, especially when salary information is kept hidden.The Wall Street Journal published an article on the good, the bad, and the downright awkward aspects of companies adopting an open salary policy. According to the article, â€Å"The idea of open pay is to get pay and performance problems out on the table for discussion, eliminate salary inequalities, and spark better performance†¦ But open pay also is sparking some awkward conversations between co-workers comparing their paychecks, and puncturing egos among those whose salaries don’t sync with their self-image.†The truth is, as employees we can make a direct correlation between our pay and how our employers perceive and value our contributions, so having this information helps take the gu esswork out of knowing where we stand- both as individuals and in comparison with our colleagues.So, despite its good intentions, when salaries are revealed employers can count on seeing a potentially disruptive effect- while those employees who are at the top of the pay scale will likely be grateful and appreciative (unless they feel that they’re still not being paid enough compared to their coworkers), those at the bottom of the salary food chain can count on being unhappy and confronting their bosses to help remedy the situation. Then, if balance regarding compensation is not reached, it will likely lead to some employees seeking better opportunities elsewhere. Making salary information public can also lead to potential awkwardness and strife among colleagues who sit at opposite ends of the compensation spectrum, which can adversely affect productivity and motivation.It seems clear that although there are some truly good potential reasons for publicly disclosing salary inf ormation, there are some significant potential pitfalls that employers should be on the lookout for when making the decision to do so. Progressive employers who react quickly and decisively to address issues regarding pay inequity will be in the best position to quell any potential disruptions, while those who are slow or late to respond may create some tension among their staff or lose some valuable talent to competitors who are willing to pay your employees what they feel they deserve.Perhaps the best approach for handling the issue of whether or not to publicly disclose salary information is to plan carefully- and proceed with caution.

How to Solve a Redox Reaction Problem

How to Solve a Redox Reaction Problem This is a worked example redox reaction problem showing how to calculate volume and concentration of reactants and products using a balanced redox equation. Key Takeaways: Redox Reaction Chemistry Problem A redox reaction is a chemical reaction in which reduction and oxidation occur.The first step in solving any redox reaction is to balance the redox equation. This is a chemical equation that must be balanced for charge as well as mass.Once the redox equation is balanced, use the mole ratio to find the concentration or volume of any reactant or product, provided the volume and concentration of any other reactant or product is known. Quick Redox Review A redox reaction is a type of chemical reaction in which reduction and oxidation occur. Because electrons are transferred between chemical species, ions form. So, to balance a redox reaction requires not only balancing mass (number and type of atoms on each side of the equation), but also charge. In other words, the number of positive and negative electrical charges on both sides of the reaction arrow are the same in a balanced equation. Once the equation is balanced, the mole ratio may be used to determine the volume or concentration of any reactant or product as long as the volume and concentration of any species is known. Redox Reaction Problem Given the following balanced redox equation for the reaction between MnO4- and Fe2 in an acidic solution: MnO4-(aq) 5 Fe2(aq) 8 H(aq) → Mn2(aq) 5 Fe3(aq) 4 H2O Calculate the volume of 0.100 M KMnO4 needed to react with 25.0 cm3 0.100 M Fe2 and the concentration of Fe2 in a solution if you know that 20.0 cm3 of solution reacts with 18.0 cm3 of 0.100 KMnO4. How to Solve Since the redox equation is balanced, 1 mol of MnO4- reacts with 5 mol of Fe2. Using this, we can obtain the number of moles of Fe2: moles Fe2 0.100 mol/L x 0.0250 L moles Fe2 2.50 x 10-3 mol Using this value: moles MnO4- 2.50 x 10-3 mol Fe2 x (1 mol MnO4-/ 5 mol Fe2) moles MnO4- 5.00 x 10-4 mol MnO4- volume of 0.100 M KMnO4 (5.00 x 10-4 mol) / (1.00 x 10-1 mol/L) volume of 0.100 M KMnO4 5.00 x 10-3 L 5.00 cm3 To obtain the concentration of Fe2 asked in the second part of this question, the problem is worked the same way except solving for the unknown iron ion concentration: moles MnO4- 0.100 mol/L x 0.180 L moles MnO4- 1.80 x 10-3 mol moles Fe2 (1.80 x 10-3 mol MnO4-) x (5 mol Fe2 / 1 mol MnO4) moles Fe2 9.00 x 10-3 mol Fe2 concentration Fe2 (9.00 x 10-3 mol Fe2) / (2.00 x 10-2 L) concentration Fe2 0.450 M Tips for Success When solving this type of problem, its important to check your work: Check to make certain the ionic equation is balanced. Make certain the number and type of atoms is the same on both sides of the equation. Make certain the net electrical charge is the same on both sides of the reaction.Be careful to work with the mole ratio between reactants and products and not the gram quantities. You may be asked to provide a final answer in grams. If so, work the problem using moles and then use the molecular mass of the species to convert between units. The molecular mass is the sum of the atomic weights of the elements in a compound. Multiply the atomic weights of atoms by any subscripts following their symbol. Dont multiply by the coefficient in front of the compound in the equation because youve already taken that into account by this point!Be careful to report moles, grams, concentration, etc., using the correct number of significant figures. Sources Schà ¼ring, J., Schulz, H. D., Fischer, W. R., Bà ¶ttcher, J., Duijnisveld, W. H., eds (1999). Redox: Fundamentals, Processes and Applications. Springer-Verlag, Heidelberg ISBN 978-3-540-66528-1.Tratnyek, Paul G.; Grundl, Timothy J.; Haderlein, Stefan B., eds. (2011). Aquatic Redox Chemistry. ACS Symposium Series. 1071. ISBN 9780841226524.

Human Development in Brazil Essay Example | Topics and Well Written Essays - 750 words

Human Development in Brazil - Essay Example Conditions are slowly improving for all Brazilians, as the Human Development Indicators(HDI) imply, though translating the figures into real progress is more difficult. The HDI is developed by the United Nations Development Programme to aid in determination of how well â€Å"countries turn income into health and educational opportunities†(UNDP). The 2006 report indicates that Brazil falls into the fifty-eighth place among one hundred seventy seven nations. The HDI is calculated by combining three sets of data: lifespan, knowledge and standard of living. The UNDP provides other indices such as the Human Poverty Index(HP1 and HP) and the Gender Related Development Index(RDI). HDI is the most inclusive and allows for a better understanding of overall data. Brazil is by no means a poor country, with abundant natural resources and $30 billion in foreign capital invested by 2002. However, â€Å"forty million live on less than $2 per day, while twenty million live on less than $1 pe r day.†(UNDP). Inequality of income is linked to gender, race and geographical region. The Northeast, where the greatest disparities exist, is comparable economically to nations such as Kenya. The UNDP has set a goal of lowering poverty to below ten percent by the year 2015. This goal seems fully attainable as the poverty rates fall from twenty percent in 1991 to roughly fourteen 2 percent by 2000. Obstacles such as slowing economic growth are identified as barriers to attaining that goal.

Analysis essay Example | Topics and Well Written Essays - 1000 words - 1

Analysis - Essay Example With this exposure a new sense of understanding and confidence is gained. The new world has its own realities and customs. It is the experience of an individual which changes reality for him as he perceives it to be. Antonio and Longoria are two individuals suffering from abrupt changes. Longoria completely changes his beliefs and principles after he joins the battalion, whereas Antonio enjoys a mundane life until his wife and son are killed by guerillas. It is this experience of life that changes the reality for Antonio. So in this theory on education, Plato argued that if one were to learn or search for truth, he ought to do so in a gradual manner (PSYOPS). The characters mentioned above were exposed to sudden and unexpected experiences and as a result they could not grasp the real meanings of such experiences and hence the way they perceived reality was changed. Characterized as an ordinary citizen under rural setting, Antonio Bernal is a Guatemalan who sought to live a life of simplicity which he managed to fulfill with a radical wife and son Carlitos in brief span, despite his feeblish attributes. Originally, he is that type of fellow who is far from engaging complex ideas and would rather prefer humble settlement and not demand beyond ordinary living at San Cristobal, Acatapan. He does not find satisfaction in complex ideas and the results that arise as a result of acting on such ideas. Little does he know that his joyful pursuits in wedlock with Elena, whose fulfillment lies further on revolutionary movements against unlawful regularities in their region, is bound to culminate into tragedy. Longoria, on the other hand, is a peasant who leaves his mother in spite of his reluctance to join the army, where he got completely altered on becoming an official member of the Jaguar Battalion. His life is completely disturbed and changed by the s udden change in circumstances that he encounters after

Evidence Based Practice for Pain Assessment

Evidence Based Practice for Pain Assessment   Introduction: Pain is a sensation of discomfort that is subjective to each individual, and it is characterized by an unpleasant feeling that can be either physiological or psychological. Acute pain is a sudden feeling of pain, occurring for a short duration lasting less than 3 months and disappearing once the injury has healed. Nurses are the most health care providers present on the unit with patients; therefore, they are the main providers responsible to carry out pain assessment appropriately. Nurses are expected to intervene accordingly to a person’s self-reported pain, and work with the person to manage the pain appropriately. Hence, nurses are required to possess the competencies to assess and manage pain, including knowledge and skills in interviewing techniques, and the ability to do physical assessment and manage pain of individuals who don’t have the ability to self –report (Herr, Coyne, McCaffery, Manworren, Merkel, 2011, as cited in RNAO, 2013). It is evident that unrelieved or poorly managed pain is a burden on the person, the health care system and society (Lynch, 2011, as cited in RNAO, 2013). In fact, 50 to 75 % of postoperative patients do not attain sufficient pain relief (Huang et al., 2001; Chung Lui, 2003, as cited in Bell Duffy, 2009) and some providers underestimate the intensity of the pain for 50% of the cases (Helfand Freeman, 2009). Therefore, this observed nursing practice gives rise to a PICO clinical question. In adult patients with acute pain, does utilizing a standard pain assessment protocol, in comparison to the current practice, affect the pain relief process? Literature review: Effective pain management is a person’s right. Hence, assessing pain, implementing interventions to alleviate it, and prevent it are priorities while caring for a person (Jarzyna et al., 2011, as cited in RNAO, 2013). The article written by Bell and Duffy (2009) inspects two important barriers that serve as obstacles for appropriate pain assessment, which are the beliefs and attitudes of patients and nurses, and time management. Research done by Sloman et al. reinforced that pain can be perceived differently in various cultures (as cited in Bell Duffy, 2009). Regarding the nurses’ attitudes, a triangulated study performed by Schafheutle et al. found that 39.3 % of respondents stated that not having enough of time, enough staff on the units and being overwhelmed with work were major features contributing to unproductive pain assessment (as cited in Bell Duffy, 2009). Regarding time management, an observational study was performed for random nurses that showed that inte rruptions, such as answering the telephones, participating in the multidisciplinary rounds, assisting other nurses and looking for things contributed in poor pain assessment practice. In addition, it was noted that nurses’ priorities were to get all tasks and activities done before the end of their shift rather than allowing time to interact directly with patients to assess their comfort and pain level (Manias et al., 2002, 2005, as cited in Bell Duffy, 2009). While assessing acute pain in adults patients, nurses have to be aware of the routine pain assessment, the choice of measure and the protocols. According to Helfand and Freeman (2009) study, there has been an agreement among most of the institutions that routine assessment of self-reported pain is the best measurement for pain assessment, since some providers underestimate the intensity of the pain for 50% of the cases. According to Helfand and Freeman (2009) study, no evidence was found that directly linked the timing, frequency, or method of pain assessment with outcomes or safety in medical inpatients. It was also noted that instituting routine pain assessment along with an educational component improved rates of assessment and treatment. The protocols in the institutions tend to guide the assessment and the management of pain; hence the assessment should be unified and accurate in order to intervene accordingly. Pain is universal but it is a subjective experience. Hence, it is challenging to obtain adequate objective information about it. Many assessment tools are used to rate and assess pain, such as the Visual Analogue Scale, the Verbal Numeric Rating Scale, Verbal Description Scales, Facial Pain Scales, Brief Pain Inventory and McGill Pain Questionnaire (Helfand Freeman, 2009). For the choice of measure, it must be simple to use by the health care providers, and easy for the patients to understand and able to respond to it (Helfand Freeman, 2009). The Visual Analogue Scale for pain assessment is used universally, however its efficacy and reliability is put to question since it may bias the results. A randomized control trial was tested over forty healthy volunteers where they were induced by thermal laser stimulations. Pain was tested during different sessions using two different visual scales; the classical pain visual analog scale (unbearable pain/ no pain), and the pleasantness visua l analog scale (very pleasant/ very unpleasant). And at same time, somatosensory evoked potentials were measured. Results showed that the thermal laser stimulations that were of low intensity were reported as painful on the visual analog scale of pain, whereas they were rated as pleasant on the visual analog scale of pleasantness. Meanwhile, following the low intensity thermal stimulation, the cerebral responses indicated the activation of only C-fibers which indicate the warm sensations that are not painful. Therefore the somatosensory evoked potential results matched with the pleasantness visual analog scale and not with the classical pain visual analog scale. This signifies that when healthy individuals rate the â€Å"no pain† using the classical visual analog scale of pain, they are more likely to rate the intensity of the stimulation and not their pain perception (Kemp, Despres, Dufour, 2012) EBP Process: Observations In hospital X, Y, Z pain assessment was observed being performed by RNs. However, in hospital X, RNs were not using a pain assessment tool to assess the pain, some were just asking if the patient was in pain or not, even though the Visual Analogue Scale was available on the floor, others for sedated patients, were squeezing the patient’s skin to check response to pain, in addition to assessment of facial expressions and vital signs (heart rate), meanwhile in hospitals Y and Z, RNs were mostly using the Numeric Rating Scale to assess for pain by asking the patient to rate the pain between 0-10, where 0 was explained to be the absence of pain and 10 to be the worst pain. In hospital X, some RNs were observed documenting the pain assessment by filling a pain flow sheet, while others were only seen to document pain assessment on the pain flow sheet if the patient was on Patient-Controlled Analgesia, meanwhile in hospital Y, RNs were observed to document pain assessment per shift b asis, whereas in hospital Z, RNs documented pain assessment only after a pharmacological intervention. In hospitals X and Y, RNs were not reassessing pain after pharmacological interventions, while in hospital Z, RNs were observed to do so. In the three hospitals, RNs were observed to inform the physician if the patient was assessed to have pain. Hospital Protocols: Pain assessment protocols were taken from 3 hospitals: X, Y Z. It is important to note that the 3 hospitals were similar in the method/system that they adopted: The American system. That is, one of the references from which the pain assessment protocol of hospital X was taken, was JCR, J.Caho, Joint Commission Resources (USA), 2003. The protocol was issued on 15/01/2011 and updated on 15/01/2013. On the other hand, hospital Y has the followings as main references: Joint Commission International standards, Hospital standards, 4th edition, January 2011, Care of patient, and 2006 Lippincott Williams Wilkins, Inc., Volume 1 (4), August 2006, p. 20-28. The pain assessment protocol is issued on January 2011 and revised on March 2012. While the pain assessment protocol of hospital Z is based on the Joint Commission International Accreditation Standards for Hospitals-5th edition, JCAHO Pain Management Standards (CAMH 2002) and The Ministry of Public Health (MOH), (2003). The protocol was i ssued on December 2006 and revised on June 2014. We can note that some references are outdated thus the protocols should be often revised to keep them equivalent with the latest evidence based practice. 3 of the pain assessment protocols stress on that pain assessment should be individualized according to the patient’s age and beliefs, values and cultural considerations. Hospital Z adds that pain assessment should be part of patient handover report. Three of the protocols state that assessment of pain should be done: Post-procedure (or within 1 hour of admission), post pharmacological and non-pharmacological interventions, with routine vital signs assessment, at time of discharge, before any planned activities (physiotherapy, stress test, post-operative ambulation). Apart from the assessment of pain, reassessment is considered as a crucial aspect, to monitor the pain level, in the 3 hospital protocols. Hospital Y mentions that prior to reassessment nurses should always refer to the literature of the analgesic agent for its peak action period. In hospital Z when pain is identified (score 2 and above), DMS-MRM-Nursing Sheets-Scale is activated while in hospital Y when pain is i dentified (score three and above), pain assessment and interventions flow sheet is activated. The scales used to assess the pain of adult patients with acute pain common in 3 hospitals is the numeric scale, where the patient is instructed to choose a number from 1 to 10 that best describes his current pain, where 0 refers to no pain while 10 refers to the worst possible pain. Another common pain assessment scale among the three hospitals is the Visual Analogue Scale, where the patient points out his/her pain level across a continuum with the extremities of no pain and worst pain. The FLACC (Face, Legs, Activity, Cry, Consolability) scale is used for critically ill, sedated and paralyzed, intubated and ventilated patients in ICU in hospital Y while it is used for children up to 3 years old in hospitals X and Z. In addition, Adult Nonverbal Scale is used for patients unable to report pain in hospital Z. Similar to the FLACC and Adult Nonverbal scales used in the 2 hospitals, hospital X uses the Behavioral rating scale (components: Face, Restlessness, Muscle tone, Vocalization, Consolability) for patients unable to self-report pain. Wong Baker Facial Grimace is a common scale in the 3 hospitals for patients who cannot communicate their pain, recommended for patients of 3 years of age and older in hospital X, while up to 7 years of age in hospital Y. Guidelines: According to the guidelines mentioned in Assessment and Management of Pain Clinical Practice Guidelines (Registered Nurses’ Association of Ontario, 2013), nurses should screen for the presence, or risk of, any type of pain upon admission, after a change in medical status and prior to, during and after a procedure. Nurses should also perform a comprehensive pain assessment using a systematic approach and appropriate, validated tools and using appropriate tools for persons unable to self-report. The nurses should take into consideration the person’s beliefs, knowledge and level of understanding about pain and pain management. Then, document the person’s pain characteristics. After implementing pain relieving measures, the guidelines state that re-evaluation is important and should be done by reassessment of the pain characteristics, and accordingly documenting the outcomes. There are some validated assessment tools, recommended to be used by the guidelines, and are the following: Faces Pain Scale Revised, Numeric Rating Score, Verbal Rating Score, Brief Inventory Short Form, and Behavioral Pain Scale (See Appendices). Proposed Change/Recommendations: Most of the nurses in the three hospitals were observed to be unfamiliar with the pain assessment protocol. Hence, it is recommended to implement frequent sessions for all nurses to inform them about the criteria of the protocol, identify any gaps, and train them accordingly. Additionally, supervision is essential on each floor to evaluate the effectiveness of these sessions. It is recommended by the guidelines that health-care professionals should participate in ongoing education opportunities to improve their knowledge and skills to be able to knowledgeably assess and manage pain (RNAO, 2013). Apart from hospital setting, the guidelines recommend that educational institutions include guidelines, assessment and management of pain into their curricula for registered nurses, and all health care providers programs to indorse evidence-based practice (RNAO, 2013). It was noted that some of the hospitals’ pain assessment policies were established on outdated references. It is hence recommended that hospitals always update their policies and base them on up-to-date EBP guidelines. In addition, it is also recommended for hospitals to establish a model of care to support inter-professional collaboration for the active assessment of pain and declare pain assessment as a strategic clinical priority (RNAO, 2013). Another common observation was that the three hospitals still used the Visual Analogue Scale, which is not among the list of recommended validated pain assessment tools mentioned in the guidelines (RNAO, 2013) and research found it to be unreliable, since patients are more likely to rate the intensity of the stimulation and not their pain perception (Kemp, Despres, Dufour, 2012). The University of Zurich and ETH Zurich in Switzerland, invented a new method for accurate pain assessment: The Pain Mouse. It is an electronic pain assessment tool that offers credible evaluation, lessening missing data and unclear markings concerning pain. The device captures the clenching reaction to pain through a pressure sensor that is connected to a portable computer (Schaffner et al., 2012). PM is recommended to be used in the near future considering that it distinguishes different levels of pain, is less time consuming, more accurate and can be used for patients with limited physical activity and vision impairment compared to the Visual Analogue Scale (VAS) (Schaffner et al., 2012). References: Bell, L., Duffy, A. (2009). Pain assessment and management in surgical nursing: a literature review. British Journal of Nursing, 18(3), 153-156. Retrieved April 4, 2015, from http://web.a.ebscohost.com.ezproxy.lau.edu.lb:2048/ehost/pdfviewer/pdfviewer?vid=6sid=ff36c8fd-ed44-444c-8182-9487d39e913b%40sessionmgr4005hid=4104 Helfand, M., Freeman, M. (2009). Assessment and management of acute pain in adult medical ‎inpatients: a systematic review. Pain Medicine, 10(7), 1183-1199. Retrieved April 10, 2015, http://web.ebscohost.com/ehost/detail/detail?vid=3sid=7b1adb63-ced7-4486-94ef-4ecc54ddc64b%40sessionmgr111hid=123bdata=JnNpdGU9ZWhvc3QtbGl2ZQ%3d%3d#db=rzhAN=2010437732 Kemp, J., Despres, O., Dufour, A. (2012). Unreliability of the Visual Analog Scale in experimental pain assessment: a sensitivity and evoked potentials study. Pain Physician, 15(5), 693-699. Retrieved on April 10, 2015 from http://www.painphysicianjournal.com/2012/september/2012;15;E693-E699.pdf Registered Nurses’ Association of Ontario (RNAO). (2013). Assessment and management of pain (3rd Edition). Toronto, ON: Registered Nurses’ Association of Ontario (RNAO). Retrieved April 4, 2015, from http://rnao.ca/sites/rnao-ca/files/AssessAndManagementOfPain_15_WEB-_FINAL_DEC_2.pdf Schaffner, N., Folkers, G., Kappeli, S., Musholt, M., Hofbauer, G.F.L., Candia, V. (2012). A new tool for real-time pain assessment in experimental and clinical environments. PLoS ONE, 7(11), art. no. e51014. Retrieved on April 10, 2015 from http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0051014

The Extent of Fidel Castro’s Achievement of His Aims

To what extent was Fidel Castro able to achieve his aims between 1959 and 1979, and what prevented him from achieving more? During Fulgencio Batista’s reign in Cuba, Fidel Castro had insistently professed his dissatisfaction with the government, and when he finally took over in 1959, he effected a government that formed the basis from which the criticism of many historians today derives. To a minimal extent, Fidel Castro was able to achieve his aims between 1959 and 1979. Revealed in the 26th of July Movement’s doctrine, Castro’s goals called for Cuba to become fully independent and purely democratic while having a just society.Castro’s goals were hindered not only by the dependence on the U. S. , but also on the Soviet Union after relations with the U. S. intensified. By the end of his reign, Castro had not upheld his initial revolutionary ideology; therefore, he did not achieve his aims for Cuba to the greater extent. In one of his aims, Castro hoped to see Cuba become independent. In order to become less dependent on foreign countries, Castro implemented an economic reform that would allow Cuba to become less dependent on the foreign countries. He wanted to create a more centralized economy by confiscating businesses, many of which were American-owned.The U. S. saw this attempt of nationalization as a direct challenge to their interests, thus they removed the sugar quota placed an embargo on Cuba, threatening to destroy the base of the Cuban economy. Cuba had now become independent from U. S. trade, but with no means self-sufficiency, Cuba turned to another group–the socialist/communist nations in the eastern hemisphere. Cuba had increased their sugar production tremendously because of Castro’s speech, â€Å"The Year of Decisive Endeavor,† in 1963 which set a production goal of 10 million tons of sugar by 1970 so that Cuba could industrialize and become more self-reliant.USSR began buying this Cuban sugar in r eturn for industrial equipment and machinery which allowed Castro to develop their countries as allies. Germany also agreed to sell the industrial necessities needed by Cuba. During these early 1960 years, Cuba quickly lost nearly all economic dependence on the U. S. , but any progress toward self-sufficiency was undermined by the developing relationship with the Soviet Union. Unlike the U. S. , the Soviet Union was not hostile to the idea of an independent Cuba and was willing to defend Cuban interest.In reality, Castro might have professed his devotion to socialism in his Marxist-Leninist speech only for the benefit of siding with the world’s socialist superpowers for military and economic support. Castro’s favoring paid off because the Soviet Union placed nuclear missiles in Cuba in 1962; however, this caused U. S. -Soviet tensions to peak when the U. S. made the threat of invading Cuba. Castro also attempted to establish Cuba’s independence by making the cha nge to a Communist state, which began distinguished officially in 1965.Although the changes were small in the early 1960s, it was not until 1976 that Cuba created a new constitution. Another aspect proving Cuban independence was the buildup of a strong military force from foreign intervention and internal opposition. Castro suspected the U. S. of military action because of their discontent with his socialist policies. Nearing the end of the first decade of his reign, Castro revealed that Cuba did not meet the 10 million ton harvest, but welcomed the shame and criticism that the failure would bring about.Throughout the years of Castro’s reign, he strived to assert Cuba as an independent nation, as demonstrated with the victory in the Bay of Pigs Invasion; however, he never fully accomplished this aim, continuing to depend on their monoculture and export-oriented economy. Castro's objective for Cuba was to uphold an uncorrupted, democratic nation. However many of his actions pr ove his ideology wrong. Within the early months after he overthrew Batista, Castro made a decision with other revolutionaries to pass the Fundamental Law of the Republic in February of 1959.By passing this legislature he gave himself more legislative power as executive of the country, adding to his power to veto due to his rank as Executive of Army. Jose Cardona was replaced from his job as Head of Legislature which allowed Castro to basically become a dictator–the opposite of a democracy. The people of Cuba lost their freedoms that citizens should have in a democracy. The government began redistributing income from the urban to the rural working class. Although it created successes such as a raise in wages, a raise in purchasing power, and a decrease in unemployment, incomes were still not all equal.Land became another freedom of the people that the government decided to take away and redistribute. Large plantation estates called Latifundios were outlawed and limited to 995 acres with the Agrarian Reform Law. The INRA enforced the ARL which hurt large American owned companies like Coca Cola and Hershey’s, contributing more animosity from the U. S. Along with the Second Law of Agrarian Reform in 1963, both reforms created an agricultural system that did not produce the same amount of crop as they did pre-1959 until the late 1960s.After forming the Constitution in 1976, Castro made a government that was not very efficient and could silence any complaint with the â€Å"red tape† effect. By reorganizing the government into the military, executive committee, and communist party, the constitution made the Cuban bureaucracies contributing to a more authoritarian-like government. More and more, Castro nationalized Cuba in every aspect and Castro, having achieved an economic stability, made a Communistic dictatorship; therefore, he did not achieve his goal for a democracy in Cuba since his own regime was corrupt.Another goal that Castro had for Cu ba from 1959 to 1979 was to create a just society. One way Castro made this society was by targeting the needs of the people and providing them with an education and healthcare system. One of the biggest issues during Castro’s reign was the country’s lack of educated revolutionaries because most of the trained workers and professionals emigrated from the island. In his 1961 speech Castro proclaimed that this year would be the â€Å"Year of Education. † To some extent he did achieve this aspect of providing immediate literacy which rose from 76% to 94% in 1979.Other improvements occurred up through this first decade of Castro’s reign such as recruiting 100,000 students, the tripling of teachers, and quadrupling of schools. However, Castro did create a curriculum that would not only provide an education limited to only basic reading and writing but also indoctrinate students with the loyalty and morals of Communism. Vilma Espin, the head of The Cuban Womenà ¢â‚¬â„¢s Federation, advocated for literacy rates in the female population while also pushing for education and healthcare as well. Through Espin’s work, Castro realized that it was larger issue, thus he passed the Family Code.This law mandated equality in marriage and legitimized divorce. This law was never really enforced but women were generally happy with the gain. Gender and racial equality was an issue with which no other country in Latin America had dealt. Castro also stressed the need for a healthcare system in Cuban society in order to create a just society. His system would implement a method that trained doctors in universities around the country. Because of forming of education and healthcare systems, Castro’s aim for social justice in Cuba was achieved to some extent.Castro was not able to uphold his ideology because limited reasons. The chief reason that may have hindered him from achieving more is the on-going tensions with the U. S. and the economic con sequences from this. If the U. S. did not have the discontent toward Cuba’s regime, Cuba would have developed the revolutionized economy that they sought. If Castro had developed relations with the U. S. and given up the socialist policies, Cuba would have avoided severe U. S. tensions and the embargo altogether. The U. S. embargo on Cuba was a major influence that hindered economic prosperity and diversified agriculture.Castro focused on manipulate Cuba from his revolutionary political aims in the 26th of July Movement. Initially, he attempts to create Cuba in the ideology of a system of social justice within the broadest concept of democracy, of freedom and of human rights. Throughout his reign Castro enacted laws and promoted policies in order to create this fully independent and democratic Cuba with a just society. To the least extent, Castro fulfilled this vision during the first two decades of the revolution, and on-going tensions with the U. S. prevented Castro from ex tending his revolutionary aim.

Essay about Gender Issues of Mesopotamia - 801 Words

Gender Issues of Mesopotamia Throughout the history of our society, women have gained a certain respect and certain rights over time. Such simple aspects of life such as getting a job, voting, and even choosing who they would like to marry are things that women have fought for, for many years. At one point, these were all things that women in America and parts of Europe had no right to. Men as a whole had suppressed women and taken control of the society. Despite mass oppression in history, women have risen in society and now posses these natural rights. Back in the days of Mesopotamia, things were quite different. Women were respected for who they were and did not have to fight to gain the rights they had.†¦show more content†¦Another example, codes 151 and 152 actually show equal responsibility between both men and women: 151. If a woman who lived in a mans house made an agreement with her husband, that no creditor can arrest her, and has given a document therefor: if that man, before he married that woman, had a debt, the creditor can not hold the woman for it. But if the woman, before she entered the mans house, had contracted a debt, her creditor can not arrest her husband therefor. 152. If after the woman had entered the mans house, both contracted a debt, both must pay the merchant. 1 This example of equal responsibility shows to an extent, how women were treated respectfully within these ancient civilizations. The woman of the house has almost as much responsibility as the man. If together they accumulate a debt, both of them are to be held responsible. Evaluating Hammurabi’s Code, and determining exactly what was expected of women is very beneficial, though it isn’t sufficient enough to find out how women were treated by men. For this, we must turn to the documentation of one’s life within this time period. The story of Gilgamesh and his epic journey is a perfect example. Gilgamesh, when he is trying to tame Enkidu in the beginning of the journey, brings him a prostitute to love him. â€Å"That is Enkidu, Shamhat, show him your breasts, show him your beauty. (Ferry 8)† Gilgamesh says to Shamhat. Within this scenario, Gilgamesh is using this womanShow MoreRelatedEssay on King Hammurabi’s Efforts to Unify Mesopotamia879 Words   |  4 PagesDuring this era, ancient Mesopotamia was under the rule of theocratic monarchies. It was believed that the sole ruler was governing specifically for the gods and catering to their wishes. Mesopotamians were polytheistic, or worshippers of multiple gods, and extremely devout in their faith. They believed that the gods determined all situations and occurrences. As a ruler, King Hammurabi found Mesopotamia to be tremendously divided. 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Race and Ethnicity in Sociology

The sociology of race and ethnicity is a large and vibrant subfield within sociology in which researchers and theorists focus on the ways that social, political, and economic relations interact with race and ethnicity in a given society, region, or community. Topics and methods in this subfield are wide-ranging, and the development of the field dates back to the early 20th century. Introduction to the Subfield The sociology of race and ethnicity began to take shape in the late 19th century. The American sociologist W.E.B. Du Bois, who was the first African American to earn a Ph.D. at Harvard, is credited with pioneering the subfield within the United States with his famous and still widely taught books The Souls of Black Folk  and Black Reconstruction. However, the subfield today differs greatly from its early stages. When early American sociologists focused on race and ethnicity, du Bois excepted, they tended to focus on the concepts of integration, acculturation, and assimilation, in keeping with the view of the U.S. as a melting pot into which difference should be absorbed. Concerns during the early 20th century were for teaching those who differed visually, culturally, or linguistically from the white  Anglo-Saxon norms how to think, speak, and act in accordance with them. This approach to studying race and ethnicity framed those who were not white Anglo-Saxon as problems that needed to be solved  and was directed primarily by sociologists who were white men from middle to upper-class families. As more people of color and women became social scientists throughout the twentieth century, they created and developed theoretical perspectives that differed from the normative approach in sociology, and crafted research from different standpoints that shifted the analytic focus from particular populations to social relations and the social system. Today, sociologists within the subfield of race and ethnicity focus on areas including racial and ethnic identities, social relations and interactions within and across racial and ethnic lines, racial and ethnic stratification and segregation, culture and worldview and how these relate to race, and power and inequality relative to majority and minority statuses in society. But, before we learn more about this subfield, its important to have a clear understanding of how sociologists define race and ethnicity. How Sociologists Define Race and Ethnicity Most readers have an understanding of what race is and means in U.S. society. Race refers to how we categorize people by skin color and phenotype—certain physical facial features that are shared to a certain degree by a given group. Common racial categories that most people would recognize in the U.S. include Black, white, Asian, Latino, and American Indian. But the tricky bit is that there is absolutely no biological determinant of race. Instead, sociologists recognize that our idea of race and racial categories are social constructs that are unstable and shifting, and that can be seen to have changed over time in relation to historical and political events. We also recognize race as defined in large part by context. Black means something different in the U.S. versus Brazil versus India, for example, and this difference in meaning manifests in real differences in social experience. Ethnicity is likely a bit more difficult to explain for most people. Unlike race, which is primarily seen and understood on the basis of skin color and phenotype, ethnicity does not necessarily provide visual cues. Instead, it is based on a shared common culture, including elements like language, religion, art, music, and literature,  and norms, customs, practices, and history.  An ethnic group does not exist simply because of the common national or cultural origins of the group, however. They develop because of their unique historical and social experiences, which become the basis for the group’s ethnic identity. For example, prior to immigration to the U.S., Italians did not think of themselves as a distinct group with common interests and experiences. However, the process of immigration and the experiences they faced as a group in their new homeland, including  discrimination, created a new ethnic identity. Within a racial group, there can be several ethnic groups. For example, a white American might identify as part of a variety of ethnic groups including German American, Polish American, and Irish American, among others. Other examples of ethnic groups within the U.S. include and are not limited to Creole, Caribbean Americans, Mexican Americans, and  Arab Americans. Key Concepts and Theories of Race and Ethnicity Early American sociologist W.E.B. du Bois offered one of the most important and lasting theoretical contributions to the sociology of race and ethnicity when he presented the concept of double-consciousness in  The Souls of Black Folk. This concept refers to the way in which people of color in predominantly white societies and spaces and ethnic minorities have the experience of seeing themselves through their own eyes, but also of seeing themselves as other through the eyes of the white majority. This results in a conflicting and often distressing experience of the process of identity formation.Racial formation theory, developed by sociologists Howard Winant and Michael Omi, frames race as an unstable, ever-evolving social construct that is tied to historical and political events. They assert that differing racial projects that seek to define race and racial categories are engaged in constant competition to give the dominant meaning to race. Their theory illuminates how race has be en and continues to be a politically contested social construct, upon which is granted access to rights, resources, and power.The theory of systemic racism, developed by sociologist Joe Feagin, is an important and widely used theory of race and racism that has gained particular traction since the rise of the BlackLivesMatter movement. Feagins theory, rooted in historical documentation, asserts that racism was built into the very foundation of U.S. society and that it now exists within every aspect of society. Connecting economic wealth and impoverishment, politics and disenfranchisement, racism within institutions like schools and media, to racist assumptions and ideas, Feagins theory is a roadmap for understanding the origins of racism in the U.S., how it operates today, and what anti-racist activists can do to combat it.Initially articulated by legal scholar Kimberlà © Williams Crenshaw, the concept of intersectionality would become a cornerstone of the theory of sociologist Patr icia Hill Collins, and an important theoretical concept of all sociological approaches to race and ethnicity within the academy today. The concept refers to the necessity of considering the different social categories and forces that race interacts with as people experience the world, including but not limited to gender, economic class, sexuality, culture, ethnicity, and ability. Research Topics Sociologists of race and ethnicity study just about anything one could imagine, but some core topics within the subfield include the following. How race and ethnicity shape the process of identity formation for individuals and communities, like for example the complicated process of creating a racial identity as a mixed-race person.How racism manifests in everyday life and shapes ones life trajectory. For example, how racial biases affect student-teacher interaction from elementary school to university and graduate school, and how skin color affects perceived intelligence.The relationship between race and the police and the criminal justice system, including how race and racism affect policing tactics and arrest rates, sentencing, incarceration rates, and life after parole. In 2014, many sociologists came together to create The Ferguson Syllabus, which is a reading list and teaching tool for understanding the long history and contemporary aspects of these issues.The long history and contemporary problem of residential segregation, and how this affects  everything from family wealth, economic well-being, education, access t o healthy food, and health.Since the 1980s,  whiteness has been an important topic of study within the sociology of race and ethnicity. Up until that point, it was largely neglected academically  because it was simply seen as the norm against which difference was measured. Thanks largely to scholar Peggy McIntosh, who helped people understand the concept of white privilege, what it means to be white, who can be considered white, and how whiteness fits within the social structure is a vibrant topic of study. The sociology of race and ethnicity is a vibrant subfield that hosts a wealth and diversity of research and theory. The  American Sociological Association  even has a webpage devoted to it. Updated  by Nicki Lisa Cole, Ph.D.